PKR implication in the cell cycle arrest and in the apoptosis induced by 5-FU.

<p>(<b>A</b>) PKR^+/+ and PKR^−/− MEFs were mock treated or treated with 5 µM of 5-FU during 48 hours. Cell cycle was analyzed by flow cytometry after staining with PI. (<b>B</b>) PKR^+/+ MEFs, PKR^−/− MEFs and eIF2α^A/A MEFs were mock-treated, treated with 10 µM of 5-FU or with 100 µg/ml of Irinotecan during 48 hours. Thereafter treated cells were trypsinized and analyzed by flow cytometry to AnnexinV positive determination. The asterisks ‘‘**” designates p<0.01 and the asterisk ‘‘*” designates p<0.05 in mutated cells versus wild type cells. (<b>C</b>) MCF-7 breast cell line expressing shRNA against PKR (shRNA-PKR) or expressing a control shRNA (shRNAc) and (<b>D</b>) colon and breast cancer cell lines SW-480, T84, MCF-7 and T47D, were mock-treated, or treated with 10 µM of 5-FU during 48 hours. Subsequently, cells were trypsinized and analyzed by flow cytometry to AnnexinV positive determination. Values shown represent the mean of the triplicate determinations calculated from a single experiment. Experiments were repeated at least three times. The asterisks ‘‘**” designates p<0.01 in T84 versus SW-480 cells and T47D versus MCF-7 cells.