DIDS inhibits HCO₃⁻ induced intracellular acidification recovery in rat caput epididymal epithelium.

<p>A: The record chart showing that DIDS inhibited the HCO₃⁻ mediated recovery for NH₄Cl induced acidification in the rat caput epididymal epithelium. The HEPES-KH solution containing 40 mM NH₄Cl caused intracellular alkalization. Wash the NH₄Cl with HEPES-KH solution with 1 mM amiloride would arouse intracellular acidification with no remarkable recovery. The recovery was still suppressed when the solution was changed into HCO₃⁻ -KH solution containing 1 mM DIDS and 1 mM amiloride; B: The summary of results showing the effects of DIDS on the HCO₃⁻ mediated intracellular acidification recovery. HCO₃⁻ mediated intracellular acidification recovery in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0022283#pone-0022283-g002" target="_blank">Fig. 2B</a> is set as control group. Data are expressed as the change of the pH value per minute. NH₄Cl, 40 mM NH₄Cl; ami, 1 mM amiloride; DIDS, 1 mM DIDS; Ctl, Control; Columns and error bars are mean ± S.E.M. (n = 5; **P<0.01 vs Ctl).