Cytokines in sera and in vitro splenocyte cultures.

<p>Serum cytokine concentrations measured at days 34 (A) and 91 (B) in TLR4 negative and TLR4 positive groups of mice. Significantly higher IL-12p70, TNF alpha, MCP-1 and IL-10 concentrations in TLR4 negative groups of mice at day 34 after immunization, while at day 91 IL-12p40, TNF alpha and MCP-1 were higher in TLR4 positive mice. (C) Interleukin17 concentrations are reduced in TLR4 deficient in vitro spleen cell cultures after 48 hours in the presence of type II collagen (100 ug/ml) or anti-CD3 antibodies (1 ug/ml)(*p<0,05).