The SCI-nu/nu mice model of CNS leukemia.

<p>(A) Clinical signs of CNS leukemia in mice. (A1, A2, arrow) The mouse developed paralysis in its left forelimb and cranial nerve with light loss. (A3, arrow) The mice developed paralysis in both fore limbs and hind limbs. (B, arrow) Neoplasms of different parts with or without intracranial hemorrhage in removed brains. (C) Infiltrations of SHI-1 examined with histopathology assay. (C1, C2, C3) Brain parenchyma pathobiology stained with H&E. (C4) Examination of bone marrow smears stained with Wright's-Giemsa. (D) Organs with infiltrations of SHI-1 examined with RT-PCR analysis of human MLL/AF6 fusion gene transcription in mice with or without GM6001 treatment. (D1) RT-PCR analysis of MLL/AF6 fusion gene of brains and bone marrow from SCI nu/nu-mice. Lanes 1–5: Human MLL/AF6 fusion genegene amplified in SHI-1 cell and in brain, bone marrow, stomach and liver of mice with CNS leukemia. lane 6–9: Human MLL/AF6 fusion gene amplified in stomach, liver, brain and bone marrow of mice with GM6001 treatment. lane 10: negative control. (D2) The number of organs with infiltrations of SHI-1 examined with RT-PCR analysis of human MLL/AF6 fusion gene transcription in mice with or without GM6001 treatment. Original magnification: (C1), ×40; (C2), ×200; (C3), ×1000; (C4), ×1000.