Immunofluorescent assay for viral protein expression in biliary epithelia of extrahepatic bile ducts on 7 dpi.

<p>Extrahepatic bile ducts infected by rotavirus expressed cytoplasmic viral proteins in the epithelial layer. siNSP4 transfection decreased the expression of NSP4, VP7 and VP4. In serial sections, NSP4 was positive but VP4 and VP7 were both negative in siVP7 transfected mice. siVP4 transfected mice had positive expression of VP7 and NSP4.