LMP1Δ BAC replicates to wildtype levels in HEK293 cells.

<p>(A) Western blot analysis of 293 cells with the indicated BAC 4 days after the virus was induced to replicate with Zta or Zta and LMP1 expression. Rta, BMRF1 and lytic protein expression was induced to the same extent in wt and LMP1Δ cells. LMP1 transcomplementation inhibited Rta expression. (B) Zta induces virus DNA replication in wt and LMP1Δ HEK293 cells, Gardella gel analysis, linear genomes marked by black bar. (C) Zta-induced replication is unaffected by LMP1 deletion; .EBV (BALF5) per GAPDH copies of intracellular virus was normalized to the percentage of gp350+ cells.