<p><b>A</b>) pH profile of CD39L2 activity with ADP or ATP at room temperature. CD39L2 has optimal pH at 5.5 and is selectively active on ADP throughout the pH range. <b>B</b>) First-order-rate constant of CD39L2 for ADP or ATP at pH 7.5. The specific rate constant of CD39L2 for ADP or ATP in the kinase assay buffer at room temperature was determined to be 41.0 or 0.7 nmol·min<sup>−1</sup> mM<sup>−1</sup>·µg<sup>−1</sup> (the slopes of the curves), respectively. <b>D</b>) Relative CD39L2 activity at different salt concentrations.</p
Item does not contain fulltextExtracellular ATP and ADP trigger inflammatory, vasodilatatory, and pr...
<p>Representative thin layer chromatography (A) shows increased dephosphorylation of 14C-labeled ADP...
Rat CD39, a membrane-bound ectonucleoside triphosphate diphosphohydrolase that hydrolyzes extracellu...
<p><b>A</b>) Rate constants for CD39L2 in the presence of different concentrations of ATP. The react...
<p>CD39L2 selectively releases the β-phosphate of the ADP generated from a kinase reaction. The rele...
<p>The kinetic data were measured in the buffer of 25 mM Tris, 150 mM NaCl, 10 mM MgCl<sub>2</sub>, ...
<p>Representative thin layer chromatography (A) shows increased dephosphorylation of 14C-labeled ATP...
<p>CD39-transfected HEK293 cells (A) and human ECs (B) as well as untreated and Lipofectamin-treated...
Kinases use adenosine-5'-triphosphate (ATP) as the donor substrate and generate adenosine-5'-diphosp...
Background The hydrolysis of ATP and ADP by ecto-nucleoside triphosphate diphosphohydrolase 1 (CD39)...
<p>At different enzyme concentrations: CHIKV-nsP2T protein was incubated at different concentrations...
<p>Ecto-ATP activity of H295R was determined by measuring the amount of [<sup>33</sup>P]-P<sub>i</su...
Phosphorylation activity of the Cyclin Dependent Kinase (CDK) family of protein kinases regulates th...
Rat CD39, a membrane-bound ectonucleoside triphosphate diphosphohydrolase that hydrolyzes extracellu...
<p><b>A</b>: Effect of pH on MnP activity. The activity of 100% was that which was measured at the o...
Item does not contain fulltextExtracellular ATP and ADP trigger inflammatory, vasodilatatory, and pr...
<p>Representative thin layer chromatography (A) shows increased dephosphorylation of 14C-labeled ADP...
Rat CD39, a membrane-bound ectonucleoside triphosphate diphosphohydrolase that hydrolyzes extracellu...
<p><b>A</b>) Rate constants for CD39L2 in the presence of different concentrations of ATP. The react...
<p>CD39L2 selectively releases the β-phosphate of the ADP generated from a kinase reaction. The rele...
<p>The kinetic data were measured in the buffer of 25 mM Tris, 150 mM NaCl, 10 mM MgCl<sub>2</sub>, ...
<p>Representative thin layer chromatography (A) shows increased dephosphorylation of 14C-labeled ATP...
<p>CD39-transfected HEK293 cells (A) and human ECs (B) as well as untreated and Lipofectamin-treated...
Kinases use adenosine-5'-triphosphate (ATP) as the donor substrate and generate adenosine-5'-diphosp...
Background The hydrolysis of ATP and ADP by ecto-nucleoside triphosphate diphosphohydrolase 1 (CD39)...
<p>At different enzyme concentrations: CHIKV-nsP2T protein was incubated at different concentrations...
<p>Ecto-ATP activity of H295R was determined by measuring the amount of [<sup>33</sup>P]-P<sub>i</su...
Phosphorylation activity of the Cyclin Dependent Kinase (CDK) family of protein kinases regulates th...
Rat CD39, a membrane-bound ectonucleoside triphosphate diphosphohydrolase that hydrolyzes extracellu...
<p><b>A</b>: Effect of pH on MnP activity. The activity of 100% was that which was measured at the o...
Item does not contain fulltextExtracellular ATP and ADP trigger inflammatory, vasodilatatory, and pr...
<p>Representative thin layer chromatography (A) shows increased dephosphorylation of 14C-labeled ADP...
Rat CD39, a membrane-bound ectonucleoside triphosphate diphosphohydrolase that hydrolyzes extracellu...