Score disease of mice upon EAE induction.

<p>EAE was induced in 7–10 weeks of age mice by subcutaneous injection with 150 µg MOG_35–55 peptide in presence of Freund adjuvant and Pertussis toxin (see Material and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0022841#s4" target="_blank">Methods</a>). Clinical symptoms were scored as follows: 0, normal; 1, weak/flaccid tail; 2, waddle; 3, moderate paraparesis; 4, severe paraparesis; 5, tetraparesis; 6, moribund. The mean clinical score was determined as the average score of all animals for a given genotype at days 10, 13, 14 and 15. Statistical significance was determined by one way ANOVA and free parametric Kruskal-Wallis test. *, <i>P</i><0.05; **, <i>P</i><0.01; ***, <i>P</i><0.001.