S1 and <i>Mung Bean</i> nuclease sensitivity.

<p>(A, B) Low M_r DNA from a URA3⁺LEU2⁺ clone derived from co-transformation of pAAVRepURA with plasmid pG.Rep68, was digested with <i>Dpn</i>I (loaded in the lane 2), <i>Mbo</i>I (loaded in the lane 3) , S1 nuclease (in the lane 4) and was analyzed on Southern Blot using URA3 probe (A) or ITR probe (B). (C) Southern blot analysis of low M_r of two URA3⁺LEU2⁺ clone derived from co-transformation of pAAVRepURA with plasmid pG.Rep68 was digested with <i>Mung Bean</i> nuclease (in the lane lane 2 and 4) and compared with the not digested DNA (lane 1, 3). DNA was detected using the URA3 probe. The arrows indicate the ssDNA which disappeared after digestion with S1 and <i>Mung Bean</i> nuclease.