H₂O₂ activation of EGFR does not induce receptor dimerization.

<p><b>A.</b> Serum-starved A549 cells were exposed (or not) to 100 ng/ml EGF or 1 U/ml GO for 15 min. Then, the cross linking reagent EDAC (1 mM) was added and incubation was continued for an additional 15 min. EGFR was IPed from cell lysates, resolved by SDS-PAGE and IBed for total and Tyr-phosphorylated EGFR as indicated; presence of EGFR dimers is indicated. <b>B.</b> Serum-starved NIH-3T3 cells over-expressing wild type EGFR were treated as in A; 50 µg of total cell lysates were IBed for total and Tyr-phosphorylated EGFR as indicated.