ROS production in macrophages from <i>Ucp2−/−</i>, <i>iNos−/−</i>, and <i>(Ucp2-iNos)−/−</i> deficient mice.

<p>Peritoneal macrophages were obtained at day 10 after MOG immunization and stimulated <i>in vitro</i> with 500 ng/ml phorbol merystil acetate during 1 hour as described in <i>Materials and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0022841#s4" target="_blank">Methods</a></i>. Data represent mean ± SEM from 5–13 mice per strain. Student's <i>t</i> test was performed to determine the statistical significance of the differences between <i>Ucp2−/−</i>, <i>iNos−/−</i> and <i>(Ucp2-iNos)−/−</i> mice. *, <i>P</i><0.05; **, <i>P</i><0.01.