Cellular immune responses induced by the DNA vaccine candidates.

<p>Intra-cellular INF gamma (IFNγ) staining IFNγ production was measured by FACS analysis after intracellular staining of IFNγ). Balb/C mice (n = 6 per group) were inoculated IM with (i) pORT ConcTat NG, (ii) pORT Gag 203 , (iii) pORT Env NG2 , (iv) pORT Env NG9 , (v) pORT gp120 C N54 (positive control), (vi) pORT1a clade (negative control). One week after the second/third booster immunization, spleen cells were isolated and tested for specific cellular immune responses by measuring IFN-g production after stimulation with pools of overlapping 15-mer peptides. Shown is the total number of CD8⁺ IFNγ⁺ cells per 30.000 CD8⁺ restimulated mouse spleen cells. A) mice 1–3, B) mice 4–6, C) merged data.