Ribavirin and oxymatrine inhibited amplification of the HCV sub-replicon in zebrafish larvae.

<p>Zebrafish larvae co-injected with prGC3N/p5BR were treated with ribavirin or oxymatrine respectively from 5-dpf to 10-dpf, followed by biological measurements. a. Conventional RT-PCR for anti-HCV effect of the study drug. Core+: the positive strand of core RNA; core-: negative strand of HCV core RNA. b. Real time RT-PCR examination for anti-HCV effect of the study drug. Core+: the positive strand of core RNA. c. Western blot assay for HCV core and NS5B proteins in the zebrafish larvae treated with ribavirin or oxymatrine. Core was detected with anti-HCV core antibody; beta-actin was with anti-beta-actin antibody (used as a control). d. Body length (mm) of the co-injected zebrafish larvae treated with the study drug (ribavirin or oxymatrine). Wild type larvae are shown as reference.