Effect of SLOB on expression of <i>dilps</i>.

<p>Relative <i>dilp2</i>, -<i>3</i>, and -<i>5</i> transcript levels in fly heads were measured by qPCR. <b><i>A, B</i></b><b>,</b> Relative <i>dilp2</i> or <i>dilp5</i> transcript levels are unchanged in <i>slob^IP1</i> fly heads. <b><i>C</i></b><b>,</b> Relative <i>dilp3</i> transcript levels are reduced in <i>slob^IP1</i> fly heads and rescued by expression of SLOB in mNSCs, but not by mutation of <i>to</i>. <b><i>D,</i></b> Relative <i>dilp3</i> transcript levels are increased in heads of flies overexpressing SLOB in mNSCs. <b><i>E,</i></b> The increase in <i>to</i> levels is abolished in <i>slob^IP1,dilp3</i> fly heads. Each graph is a summary of a minimum of three independent experiments (mean ± SEM). *** indicates p<0.001, One-way ANOVA with Bonferroni post-test (<i>C, E</i>) or Student's t-test (<i>D</i>).