AsA inhibits VEGF-stimulated cell growth and capillary tube formation in HUVEC.

<p>HUVEC were grown under 0.5% serum conditions and treated with or without VEGF (10 ng/mL) and various doses of AsA (5–20 µM) for 12 h as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0022745#s4" target="_blank">Materials and Methods</a>. After 12 h, both adherent and non-adherent cells were collected and processed for determination of total cell number (<b>A</b>) and dead cells percentage (<b>B</b>) as mentioned in ‘<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0022745#s4" target="_blank">Materials and Methods</a>’. <b>C.</b> HUVEC with or without VEGF (10 ng/mL) in 0.5% serum media were placed in 24-well plates coated with Matrigel and treated with AsA at indicated doses. After 10 h tubular structures were photographed at 100x magnification and tube length was measured as detailed in ‘<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0022745#s4" target="_blank">Materials and Methods</a>’. These results (A–C) were similar in 2–3 independent experiments. Each bar is representative of mean ± standard deviation of three samples for each treatment. *, p≤0.001; #, p≤0.01.