The effect of C5-deficiency on expression of hypertrophic gene expression program in response to <i>C. albicans</i> infection.

<p>Semi-quantitative RT-PCR was used to determine the levels of the indicated genes in heart RNA from <i>C. albicans</i>-infected B6, BcA17, A/J, C5aR^−/− and BALB/c mice (B6 inf, BcA17 inf, A/J inf (<b>A</b>) and B6 inf, C5aR^−/− inf, and BALB/c inf (<b>B</b>). Transcript levels in the infected (A: B6 n = 10, BcA17 n = 11, A/J n = 12; B: B6 n = 11, C5aR^−/− n = 11, BALB/c n = 12) hearts were calculated relative to those in un-infected B6. The figure represents data from at least two independent experiments. The error bars indicate the standard errors of the means. Statistically significant differences with <i>Candida</i>-infected B6 (A) or C5aR^−/− (B) hearts are indicated by asterisks (* p<0.05, **p<0.01 and *** p<0.001). P-values for the change in gene expression upon infection in a given strain (Strain Inf vs Strain) or between strains (Strain 1 chg vs Strain 2 chg) is tabulated with statistically significant changes in bold.