Identification of pancreatic cell types expressing Netrin-4.

<p>PCR analysis (<b>A</b>) and Western blotting (<b>B</b>) on select pancreatic cell populations show significant levels of Netrin-4 expression in adult primary ductal cells, ductal cell line CAPAN-1, and fetal pancreatic cells, and low levels in pancreatic islets. Representative of n = 3. SYBR green qPCR (<b>C</b>) for Netrin-4-specific transcripts in primary microvascular endothelial cells (hMEC), fetal and adult pancreatic ductal cells, and intact adult islets. SYBR green qPCR for the endothelial-specific cell adhesion molecule VE-cadherin (<b>D</b>) showing that resident endothelia cells are positioned within fetal and adult islets. Fluorescence-activated cell sorting of a single cell suspension from isolated human fetal islets immunostained for insulin (<b>E</b>), and SYBR green qPCR analysis for insulin, Netrin-4, and VE-cadherin (<b>F</b>). Data presented in C, D, E and F are representative of n = 3, with each SYBR green qPCR reaction performed in duplicate.