Transcription activity of AP-2α is downregulated by Aurora-A.

<p>(A) The summary of the transcription factors with significant changes in their activities by transcription factors array when Aurora-A was overexpressed. (B) Fluorescence images of the transcription factors arrays hybridized with nuclear extracts from KYSE150/GFP-Aurora-A and KYSE150/GFP cells. The analysis of arrays was replicated twice, exchanging the Cy3- and Cy5- label. Magnified panels show the spots representing the activity of AP-2 transcription factor that was down-regulated upon Aurora-A overexpression. (C) The summary of the AP-2α-regulated genes with significant down-expression when Aurora-A was overexpressed by mRNA expression profiling array. The black bars represent the AP-2α-regulated genes, and the gray bars represent the genes which were not the AP-2α-regulated genes. (D) The nucleoprotein extraction of KYSE150/GFP-Aurora-A and KYSE150/GFP cells were incubated with AP-2α binding DNA double-stranded oligonucleotidic probe on multi-well plates, then anti-AP-2α antibodies and the peroxidase-conjugated anti-mouse IgG were subsequently added. At last, the samples could be read for the colorimetric detection. (E) KYSE150/GFP-Aurora-A and KYSE150/GFP cells were cotransfected with (AP-2)₃-TK-LUC expression vector and a pRL-SV40 expression vector. Transcription activities were expressed as luciferase values after normalization. All experiments were performed three times and described as mean ± SD.