ACE2 overexpression in the PVN decreases NADPH oxidase activity in ACE2^−/y mice.

<p>NADPH oxidase activity in HT (A) and VLM (B) was significantly higher in ACE2^−/y mice on baseline (*<i>P</i><0.05 vs. NT, n = 4). This enzyme activity was significantly increased in NT following AngII infusion (*<i>P</i><0.05 vs. NT, n = 4) and remained more elevated in ACE2^−/y (†<i>P</i><0.05 vs. NT+AngII). Ad-ACE2 expression in the PVN of ACE2^−/y significantly decreased NADPH oxidase activity (‡<i>P</i><0.05 vs. KO+AngII+Ad-GFP). Baseline SOD activity was significantly higher in ACE2^−/y mice (C,D; *<i>P</i><0.05 vs. NT, n = 4). AngII infusion did not change SOD activity in either genotype (<i>P</i>>0.05, n = 4). Ad-ACE2 expression in the PVN of ACE2^−/y decreased SOD activity in HT (C; ‡<i>P</i><0.05 vs. KO+AngII+Ad-GFP) without changing its activity in VLM (D; <i>P</i>>0.05, n = 4).