The ratios of SAP to DNA decrease in SLE murine model.

<p>6-week-old female BALB/c mice were immunized subcutaneously with ALD-DNA, UnALD-DNA, or PBS (n = 15) for 3 times in 4 weeks. (A) The dynamics of SAP level in serum of mice immunized with ALD-DNA, UnALD-DNA, or PBS were determined by ELISA assay every 2 weeks. (B) The dynamics of circulating DNA level in serum of mice immunized with ALD-DNA, UnALD-DNA, or PBS were determined using a PicoGreen DNA detection kit (Invitrogen) every 2 weeks. (C) SAP levels in the serum of SLE murine model and controls were tested by ELISA at week 8 after initial immunization. n = 15. (D) Circulating DNA levels in the serum of SLE murine model and controls were determined using a PicoGreen DNA detection kit at week 8 after initial immunization. n = 15. (E) The correlation between SAP and DNA levels in ALD-DNA-immunized lupus mice. n = 15. Pearson correlation analysis was used to carry out the correlation study. r = 0.76; <i>P</i><0.05. (F) The ratios of SAP to DNA in SLE murine model and controls. Data are means ± SD from 15 mice in each group. ***, <i>P</i><0.001. (G) The correlation between the ratio of SAP to DNA and anti-dsDNA IgG level in SLE murine model. Pearson correlation analysis was used to carry out the correlation study. r = 0.90; <i>P</i><0.001.