Response of the P<i>asr</i>::<i>gfp</i> transcriptional fusion during macrophage infection.

<p>WT pP<i>asr</i>::<i>gfp</i> was used to infect RAW264.7 macrophages. At various times post-infection (pi) host cells were lysed, bacteria were fixed with paraformaldehyde and collected. Fluorescence produced by intracellular WT pP<i>asr</i>::<i>gfp</i> 1, 5 and 8 hours post-infection was analyzed by flow cytometry (A). The same analysis was performed when host cells were treated with 100 nM bafilomycin A1, which inhibits SCV acidification (B). Typical graphs representative of several experiments are shown.