Localization of ATP synthase β on the external membranes and in the cytoplasm of midgut cells of the vector <i>Euscelidius variegatus</i>.

<p>Whole permeabilised guts, as observed in light microscopy (A), are strongly labelled by the antibody against ATP synthase β (B), while preimmune serum treated samples show no signal (C). Sections of permeabilized guts (D, E) reveal an intense labelling of mitochondrial ATP synthase β in the cytoplasm (arrows). In sections of not permeabilised guts (F), ATP synthase labelling is present on the cell surface. Double immunofluorescence labelling of both ATP synthase β (G) and flotillin 1 (H) shows the coincidence of the respective signals, resulting in the yellow colour of the merged image (I). n: nucleus; gl: gut lumen. Panel E is a magnification of panel D.