Neurogenesis in the adult gerbil hippocampus after global ischemia.

<p>Animals were subjected to 5 min of global forebrain ischemia followed by reperfusion. BrdU was administered twice daily 6–9 days after ischemia. Brain sections from a control animal (A, D, G), and from an animal 14 days (B, E, H) or 28 days (C, F, I, K, L) after ischemia were stained for BrdU immunoreactivity (green), for specific neuronal markers (NF-200 and NeuN -red) and astrocytic marker (GFAP-red) in the DG (A, B, C) and the CA1 (D–I). J, K, L represent magnification (z –stacks) of the picture C, F, I, respectively. Note co-localization of BrdU with mature NeuN-positive neurons in SGZ, SG and hilus at 14 days after ischemia (B) and increased number of BrdU/NeuN positive cells in SG at 28 days after ischemia (C, J). Photomicrographs are representative of observations made from six animals per time point. Scale bar 10 µm. Abbreviations: s.g.z - subgranular zone, g.z.- granular zone, h - hilus, p.l.- pyramidal layer.