Effect of SB-3CT, GM6001 and doxycycline on the growth and proliferation of HUCB-NSCs.

<p>Equivalent numbers of HUCB-NSCs were plated on fibronectin-coated coverslips and grown 8 days in serum-free medium with or without SB-3CT (10 µM), GM6001 (25 µM) or doxycycline (60 µM). A) Graph shows an average number of surviving cells after 4, and 8 days in culture. The addition of SB-3CT, GM6001 or doxycycline to the incubation medium decreased the number of surviving cells. The results (mean values +/− SEM) represent five independent experiments. One-way ANOVA and Bonferroni test: **p<.01; ***p<.001, between treatments (inhibitors vs control value). B) Graph shows the rate of HUCB-NSCs proliferation expressed as a % of control of Ki67- immunopositive cells. The present of SB-3CT, GM6001 or doxycycline in the culture reduced markedly the number of proliferating cells. The results (mean values +/− SEM) represent five independent experiments. One-way ANOVA and Bonferroni test: ***p<.001, between treatments (inhibitors vs control value).