CL075, but not pT-ODN induces changes in microglia morphology.

<p>Primary cortical cultures from wildtype mice were stimulated with 20 µM CL075 and/or 1 µM pT-ODN. After 48 hrs, cells were fixed and then stained for the microglia/macrophage marker, Iba1 using an Alexafluor-488 conjugated anti-rabbit antibody for detection. Iba1 positive cells in (A) mock (unstimulated) and (B) pT-ODN-stimulated cultures were primarily elongated with only a few amoeboid cells. In contrast, Iba1 positive cells in (C) CL075 or (D) CL075/pT-ODN stimulated cultures were completely amoeboid in appearance. However, Iba1 positive cells from (E–F) TLR7-deficient mice did not take on the amoeboid appearance after stimulation with (E) CL075 or (F) CL075/pT-ODN. Images are representative of cells in each well and are the results of one of two replicate experiments.