The environment of the PerC results in increased expression of IFNγ by CD4⁺ T cells.

<p>CXCR3⁺ CD62L^low CD44^high, CXCR3⁻ CD62L^low CD44^high and CXCR3⁻ CD62L^high CD44^low CD4⁺ T cells were sorted from Sp of naïve C57BL/6 mice and stained with CFSE. Then, cells were transferred to naïve recipient animals by i.v. or i.p. rout and re-isolated after 24 h. Cells were re-stimulated with ionomycin, PMA and brefeldinA, stained for (A) IFNγ, (B) IL-4 and (C) IL-17 production and further analyzed by flow cytometry. The percentage of cells producing the different cytokines was subsequently determined. Similar results were obtained in 2 independent experiments. The differences were statistically significant at P<0.01 (*).