HBGA-binding outcomes of various mutant P particles with single amino acid mutations in the HBGA-binding interface of VA207.

<p>Binding of wild type (A) and mutant P particles (B to G) to a panel of synthetic oligosaccharides representing HBGAs of H1, H2, H3, A, B, Le^a, Le^b, Le^x, Le^y, 3′sLe^a, and 3′sLe^x (X-axes). Y axes indicate the optical densities at 450 nm (OD₄₅₀) that were the average values of quintuplicate (A) and triplicate (B to G) experiments, respectively. Concentrations of the P particles were 10 µg/ml.