Vaccination of mice with NE-RSV attenuates disease following intranasal challenge with live RSV.

<p>Immunized mice were vaccinated intranasally (i.n.) twice at day 0 and day 28 with NE-RSV (10⁵ PFU equivalent). Control and vaccinated mice were challenged at day 56 (i.n.) with 10⁵ PFU live RSV. In (A), the expression of virus transcripts and were determined at day 4 post-infection via QPCR of lung RNA. (B) depicts representative histology (Periodic Acid Schiff's, PAS; Hematoxylin and Eosin, H&E) from control RSV infected and NE-RSV vaccinated mice at day 8 post-infection. In the top two panels, magnification bars are 200 µm in length and 50 µm in the bottom two panels. Arrows indicate airway-associated eosinophils in control RSV infected mice. In (C), the expression of <i>Muc5ac</i> and <i>Gob5</i> were assessed at day 8 post-infection via QPCR of lung RNA.