Protein levels of IL-11 affect IL-11 mRNA expression.

<p>(A) <i>In vivo</i> administration of anti-IL-11 antibodies leads to a selective down-regulation of IL-11 mRNA. The level of expression was quantified in 5 individual mice per group, using qrt-PCR and normalization against the level of GAPDH expression. Results obtained in 1 of 2 similar experiments are expressed as mean ± SEM (for IL-11 expression <i>P</i> = 0.021, for other cytokines <i>P</i>>0.05). (B) Introduction of 100 ng/ml rIL-11 in cultures of lung cells up-regulates the expression of IL-11 mRNA. Results of two similar experiments are expressed as mean of 3 wells ± SEM (<i>P</i><0.01, ANOVA, compared to negative controls and cultures stimulated with 10 ng/ml IL-11).