Expression of thioredoxin (TRx) in the neural retina.

<p>Top: Comparison of expression of TRx normalized to levels of GAPDH in rats treated with/without compounds <b>4</b> and <b>8</b> suggest that treatment with multi-functional compounds reduce induction of TRx. Retinal protein homogenate was subjected to Western blotting with anti-TRx and anti-GAPDH antibodies. Bottom: Lanes 1, control diet, non light-damaged; Lane 2, control diet, light-damaged; Lane 3, <b>4</b>-treated, non light-damaged; Lane 4, <b>4</b>-treated, light-damaged; Lane 5, <b>8</b>-treated, non light-damaged; Lane 6, <b>8</b>-treated, light-damaged. Lanes of interest were cropped from entire western blot scan prior to be auto leveled using Adobe Photoshop and analyzed by ImageJ software. Mean ± S.D.; n = 6. An asterisk (*) denotes a significant difference (p<0.05) when compared to the untreated, non light-damaged group. A double dagger (‡) denotes a significant difference (p<0.05) when compared to the untreated, light-damaged group.