Effect of LPS on alveolar macrophage and ATII cell TLR-4 protein expression.

<p>Alveolar macrophages (A) and ATII cells (B) were exposed to LPS (100 ng/ml) over a three hour time course. Cells were fixed in methanol at 0, 15 mins, 30 mins, 1 hr, 2 hrs and 3 hrs and stained with FITC-labelled anti-TLR4 antibody. TLR4 expression was visualised using confocal microscopy. Images were taken through the centre of the cell to visualise cytosolic as well as cell surface expression. Data representative of 3 separate subjects.