Effect of H₂S on CoCl₂-induced COX-2/PGE₂ overexpression.

<p>HaCaT cells were incubated with 500 µM CoCl₂ for 6 h in the absence or presence of pretreatment with NaHS at the indicated concentrations for 30 min. (<b>A</b>) Cell lysates were subjected to Western blot analysis using COX-2- or COX-1-specific antibody. (<b>B</b>) The intensity of the protein bands of a typical experiment was quantified with Image J 1.41o software. (<b>C</b>) PGE₂ level in cell supernatants was measured by ELISA. Data were shown as the mean ± SE. ^*<i>P</i><0.01 compared with control group. ⁺<i>P</i><0.01 compared with CoCl₂ treatment group.