Cas proteins negatively regulate E-cadherin expression in MCF7 cells.

<p><b>A</b>. Western analysis of MCF7 cells transfected with plasmids including vector pcDNA-HA (-), pcDNA-HA-NEDD9 (N), pcDNA-HA-BCAR1 (B), or pcDNA-HA-NEDD9 and pcDNA-HA-BCAR1 (NB). <b>B</b>. Western analysis of MCF7 transfected with siRNAs including scrambled control (scr), or targeting NEDD9 (siN), BCAR1 (siB) and both (siNB), probed with antibodies indicated. <b>C</b>. Graph represents total levels of E-cadherin normalized to β-actin. *, P<0.01, **, P<0.001. Error bars represent SE. Additional siRNA experiments (not shown) were performed with alternative siRNA oligonucleotides targeting NEDD9 and BCAR1: although knockdown was not as efficient, qualitatively similar results were obtained in regard to E-cadherin expression.