Inhibition of <i>A. fumigatus</i> internalization by PLD chemical inhibitors.

<p>A549 cells were incubated for 30 min with 1% (v/v) 1-butanol or tert-butanol (A, B), 2 nM VU0359595 (PLD1-specific inhibitor), 100 nM VU0285655-1 (PLD2-specific inhibitor), or both (C, D). Subsequently, the cells were infected with <i>A. fumigatus</i> 13073 swollen conidia at an MOI of 10. <i>A. fumigatus</i> internalization was analyzed by the nystatin protection assay (A, C) and PLD activities (B, D) were measured. Differences in [³H] PtdEtOH formation or <i>A. fumigatus</i> internalization between the untreated (control) cells and inhibitor-pretreated cells were compared. Data are represented as the mean ± SE (n = 3–4). *P<0.05.