siRNA silencing of PREP1 and PBX2 in transfected U87-MG cells differentially affects promoter activity of the -2578 A and G alleles.

<p><b>A,B.</b> Luciferase expression in U87-MG cells transfected with either the -2578 A pGL4.11 plasmid or the -2578 G pGL4.11 plasmid along with indicated siRNAs. <b>C.</b> Luciferase expression in U87-MG cells transfected with either the -2578 A pGL4.11 plasmid or the -2578 A<i>m</i> pGL4.11 plasmid. <b>D.</b> Luciferase expression in U87-MG cells transfected with either the -2578 G pGL4.11 plasmid or the -2578 G<i>m</i> pGL4.11 plasmid. All results were normalized to Renilla luciferase values. Results are expressed as the mean ± S.E. of three independent experiments carried out in duplicate. Differences between multiple groups were tested using ANOVA (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0022052#pone-0022052-g003" target="_blank">Figure 3A</a>, P = 0.0495 and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0022052#pone-0022052-g003" target="_blank">Figure 3B</a>, P<.0001). Subsequent pair wise comparisons of groups with each treatment against the control group utilized the Bonferroni correction. Student's two-sided <i>t</i> tests were used for 3C and 3D.