Interference of endogenous PLD host cell expression reduces the internalization of <i>A. fumigatus</i>.

<p>A549 cells were transfected with non-specific small interfering RNAs (siRNAs) (Control), PLD1-specific siRNAs (A, C), or PLD2-specific siRNAs (B, D). After 48 h, cells were infected with the swollen conidia of <i>A. fumigatus</i> 13073 at an MOI of 10. <i>A. fumigatus</i> internalization was analyzed by the nystatin protection assay (A, B) and PLD activities (C, D) were measured. Differences in [³H] PtdEtOH formation and <i>A. fumigatus</i> internalization between the untransfected cells (Control) and PLD-silenced cells were compared. Data are represented as the mean ± SE (n = 3–4). *P<0.05. The immunoblots represent PLD1 and PLD2 expression in A549 cell lysates.