Analysis of intratumoral cytokine and chemokine expression.

<p>At day 28, TC-1 and MMC tumors from Tx(Mock) and Tx(I-LV-aCTLA4)+Dox were harvested. <b>A</b>) qRT-PCR for TGF-β RNA. Anti-TGF–β RNA was equalized to levels of GAPDH mRNA measured in parallel in each sample. Shown is the fold increase in Tx(I-LV-aCTLA4)+Dox vs Tx(Mock) groups in the TC-1 model. N = 3. The outcome in the MMC model was similar. <b>B–C</b>) cytokine proteome array. Tumor lysates were pooled and analyzed for the indicated cytokines using the Proteome Profiler from R&D Systems. <b>B</b>) Assembled images of dot blots for key cytokines from MMC and TC-1 tumors. <b>C</b>) (TC1) and <b>D</b>) (MMC). The plots were scanned and signal intensity was measured. BLC = CXCL13, I-309 = CCL1, eotaxin = CCL11, IP-10 = CXCL10, JE = CCL2, MIG = CXCL9, MIP1α = CCL3, MIP1β = CCL4, RANTES = CCL5, SDF-1 = CXCL12, TARC = CCL-17. The array includes positive controls provided by the manufacturer.