Glycerol hinders the liposome clustering activity of sMunc18-1.

<p>(<b>A,B</b>) DLS measurements of particle size in samples containing 15% glycerol, protein-free liposomes (POPC∶DOPS 85∶15 molar ratio; 30 µM lipids) and 4 µM sMunc18-1 right after mixing (<b>A</b>) and after 1 hr incubation at 37°C (<b>B</b>). The average radius (R_av) and polydispersity (Pd) are indicated.