Treatment of EAE mice with RTL551 inhibits expansion of CD44 expressing CD4+ T-cells in the periphery compared to treatment with Vehicle or RTL550.

<p>Splenocytes and lysed blood cells were stained with respective antibodies and subjected to flow cytometry. Dot plot for CD44 staining on blood CD4+ T-cells on day 3 post onset is shown in panel A. Cells were gated on live CD4+ cells for this analysis. Values in panels B & C are representative of 8 mice per group on D3 after onset of clinical signs of EAE except for naïve which had 5 mice. Significant differences between the groups (p≤0.05) were determined using Student's <i>t</i> test and are indicated by brackets.