Growth of B31-A3 and CP176 in modified enriched medium.

<p><b>A</b>) BSK-lite containing GlcNAc as the sole carbohydrate. •, B31-A3; □, CP176. Left panel, 25°C: B31-A3 achieved a stationary phase cell concentration of 1.8×10⁸ and CP176 achieved a stationary phase cell concentration of 1.5×10⁸. Right panel, 37°C: B31-A3 achieved a stationary phase cell concentration of 8.5×10⁷ and CP176 achieved a stationary phase cell concentration of 6.3×10⁷. All data points were determined in quadruplicate. Results are representative of two independent experiments. <b>B</b>) BSK-lite containing GlcNAc and glycerol as the principal carbohydrate. •, B31-A3; □, CP176. Left panel, 25°C: B31-A3 achieved a stationary phase cell concentration of 6.4×10⁸ and CP176 achieved a stationary phase cell concentration of 1.1×10⁸. The difference in stationary phase cell concentration between B31-A3 and CP176 at 25°C was significant (<i>P</i><.001). Right panel, 37°C: B31-A3 achieved a stationary phase cell concentration of 9.1×10⁷ and CP176 achieved a stationary phase cell concentration of 6.4×10⁷. All data points were measured in quadruplicate. Results are representative of three independent experiments. Error bars indicate SEM.