Capillary growth kinetics vary by stromal cell type.

<p>A–C) Representative 4x images of fluorescently-tagged HUVECs forming capillary networks in co-culture with A) AdSCs, B) MSCs, C) NHLFs. D) Comparison of growth kinetics for several stromal cell types in the bead assay. “Mono” denotes the stromal cells were cultured as a monolayer on top of the fibrin gel, a fixed distance away from the HUVEC-coated beads; “distributed” indicates the stromal cells were distributed throughout the entire 3D gel. E) Comparison of the number of vessel segments per network for each stromal cell type. The * symbol indicates statistical significance (p<0.05) between time points within a given culture condition. The +, #, %, and @ symbols indicate statistical significance (p<0.005) for a particular culture condition at a given time point versus the other culture conditions. For example, the total lengths of the capillary networks induced by distributed NHLFs are significantly different than those induced by the monolayer NHLFs and those induced by all of the other cell types and distributions (denoted by #).