Culture of DH01 cells to post-confluence prior to transplantation induces apoptosis and necrosis.

<p>(<b>A</b>) Representative flow cytometry dot plots for annexin V (AnV) and propidium iodide (PI) showing that transplant conditions (TC) did not affect cell viability compared to baseline conditions (BC). However, overall levels of late apoptosis (AnV⁺, PI⁺) and necrosis (AnV^-, PI⁺) increased in cell suspensions prepared post-confluence. (<b>B</b>) Graphical representation of the levels of nonviable cells presented as early apoptotic (AnV⁺/PI^-, white), late apoptotic (AnV⁺/PI⁺, grey) and necrotic (AnV^-/PI⁺, black). Error bars reflect standard deviation of the total (0.5–20 represent numbers of cells seeded x10⁶ on Day 0; BC = baseline conditions; TC = transplant conditions; NS = not significant; *Significant at p<0.05; ***Significant at p<0.001).