Sialidase localization and interaction with GpdC.

<p>(A) Autoradiography of ³H-palmitate labeled sialidase in different bacteria. (B) Detection of sialidase by western blot analysis (anti-SiaC antibody) in total cell extracts (TC) and outer membrane protein (OMP) fractions of <i>Cc5</i> wt and <i>ΔsiaC</i> bacteria complemented with the soluble periplasmic sialidase (SiaC_C17Y). (C) Number of divisions after 23 hours growth on HEK293 cells of <i>ΔsiaC</i> bacteria expressing SiaC or SiaC_C17Y. (D) Fetuin glycosylation state after 2 hours of incubation in the presence of the different strains, determined by staining SNA. (E) Same as D, analyzed by western blot with anti-fetuin antibodies. (F) Co-purification of SiaC with GpdC-Strep-His produced in a <i>ΔgpdCDGE</i> background. GpdC was detected with anti-Strep antibody and SiaC with anti-SiaC antibodies.