Congo Red spectroscopic assay.

<p>Time-dependence of Congo Red (CR) absorption when CR is bound to Aβ and to Aβ-metal complexes, in the presence and in the absence of resveratrol. The reaction mixtures containing 1,5 µM Aβ or Aβ-metal complexes, 0.1 M Tris/HCl, 0.15 M NaCl, pH 7,4 and 0 (solid line) or 45 µM (dash) resveratrol, were incubated at room temperature for the indicated times. The absorbance due to CR was subtracted. Each point represents means ± SD of three individual experiments.