Lentiviral transduction of DC-SIGN-expressing 293T cells.

<p>(A) Expression of DC-SIGN in 293T (solid fill) and 293T.DCSIGN (open fill) was detected by flow cytometry. (B) Transduction titer of lentiviruses were quantified by serially diluting fresh viral supernatants of FUGW/SFVG and FUGW/VSVG and used to transduce 2×10⁴ 293T.DC-SIGN (open bars) or parental 293T cells (solid bars). Three days later, the transduction efficiency was measured by analyzing GFP expression using flow cytometry where the corresponding viral titer was calculated. Values are given as the mean of triplicates ± S.E.