Cocaine increased ΔFosB protein levels in the dorsal striatum and nucleus accumbens.

<p>A) Representative western blots with three FosB antibodies: 5G4, sc-48, H-75. Due to its specific and robust labeling for ΔFosB isoforms (34–37 kD), 5G4 was used for subsequent experiments. B) Representative western blot of dorsal striatum samples from 2-week cocaine- or saline-treated males and females probed with 5G4 anti-FosB. The same blot reprobed for actin is shown below. Bar graph shows quantification of optical density from n = 3 animals/group. C) Representative western blot of nucleus accumbens samples from 2-week cocaine- or saline-treated rats probed with 5G4 anti-FosB. The same blot reprobed for actin is shown below. Quantification of optical density from n = 3 samples/group. Two-way ANOVAs showed that cocaine significantly increased ΔFosB in both dorsal striatum (B) and nucleus accumbens (C).