Transduction of MoDCs by lentiviruses is inhibited with anti-DCSIGN antibody and mannan.

<p>Human monocyte-derived DCs (MoDCs) were generated by culturing respective precursor cells in the presence of GM-CSF and IL-4. (A) The adherent cells (1×10⁶) were cultured for 2 days and then DC-SIGN expression was detected by flow cytometry. (B) Human MoDCs (1×10⁶) were incubated for 1 hour with mannan (200 µg/mL), anti-DC-SIGN(R) antibody (20 µg/mL) or without any reagents. The cells were then infected with FUGW/SFVG or FUGW/VSVG (MOI = 10) for 8 hours in the presence of blocking reagents. GFP expression was assayed by flow cytometry five days post-transduction where one representative figure is shown with values given as the mean of triplicates ± S.E.