Presence of an excess of unprocessed gp160 in PSV but not MC HIV-1 virion preparations.

<p>(<b>A</b>) SDS-PAGE of HIV-1_LAI-JR-CSF PSVs (pcDNA) showing excess of uncleaved gp160, and HIV-1_LAI-JR-CSF MC (pLAI; sequence matched in Env) showing only the much fainter gp120 band, as well as gp41. Virus loaded was normalized by p24 ELISA. (<b>B</b>) BN-PAGE of samples in panel A, showing mostly oligomeric Env (PSVs and MCs), with HIV-1_LAI-JR-CSF PSVs showing greater heterogeneity in staining with the gp120 mAb cocktail, and with MCs showing much less abundant, but mainly trimeric Env. Input virus was normalized as in <b>A</b>.