<p>(<b>A</b>) mRNA expression of insulin receptor isoform A and B in human astrocytes. Given are means + SEM, N = 3. * = there were significant differences between groups (Student's t-test, p = 0.0181). (<b>B</b>) mRNA expression of GLUT2 in human astrocytes (black bar), human myotubes (white bar), human adipocytes (dark grey bar), and HepG2 (human hepatocellular carcinoma cell line, light grey bar). mRNA expression was normalized for mRNA of the housekeeping gene <i>Rps13</i>. Bars represent means + SEM. N = 3. There were significant differences between the groups (ANOVA, p = 0.0006). * indicates significant difference from astrocytes (Tukey Kramer post hoc test p<0.05).</p
<p>Expression was lower in KO compared to WT astrocytes for all three genes studied. n = 9. ** p<0.0...
<p>Western blot for phosphorylated (P∼p42/44<sup>mapk</sup>) or total (p42/44<sup>mapk</sup>) p42/44...
Reduced IGF1R but not downstream signalling in human astrocytes derived from temporal lobe resection...
<p>(<b>A</b>) Insulin receptor, (<b>B</b>) IRS-1, (<b>C</b>) IRS-2, (<b>D</b>) GLUT1, (<b>E</b>) GLU...
<p>(<b>A</b>) Equal amounts of total cell lysates of two independent experiments were loaded onto a ...
In the human brain, there are at least as many astrocytes as neurons. Astrocytes are known to modula...
BACKGROUND: The insulin/IGF1 signalling (IIS) pathways are involved in longevity regulation and are ...
Two insulin receptor mRNA species are expressed in human tissues as a result of alternative splicing...
Impairment of insulin signalling using a combined insulin-fructose treatment protocol over a longer ...
<p>Western blot analysis was used to detect phosphorylated insulin receptor (p-IR) b, total IRb, ins...
<p>(<b>A</b>) The indicated cells were preincubated with the PI-3 kinase inhibitor LY294002 for 1 ho...
<p>A. Relative expression of exogenous <i>Zip7</i> mRNA. B. Relative expression of endogenous and ex...
Mature adipocytes originate from fibroblast-like precursor cells, preadipocytes, which differentiate...
Functional impact of impaired insulin signalling. (A) Human astrocytes treated with ± 1 μM insulin ±...
<p>A: RNA yield (picograms per cell) from the sorted islet cell fractions, expressed as mean + SEM (...
<p>Expression was lower in KO compared to WT astrocytes for all three genes studied. n = 9. ** p<0.0...
<p>Western blot for phosphorylated (P∼p42/44<sup>mapk</sup>) or total (p42/44<sup>mapk</sup>) p42/44...
Reduced IGF1R but not downstream signalling in human astrocytes derived from temporal lobe resection...
<p>(<b>A</b>) Insulin receptor, (<b>B</b>) IRS-1, (<b>C</b>) IRS-2, (<b>D</b>) GLUT1, (<b>E</b>) GLU...
<p>(<b>A</b>) Equal amounts of total cell lysates of two independent experiments were loaded onto a ...
In the human brain, there are at least as many astrocytes as neurons. Astrocytes are known to modula...
BACKGROUND: The insulin/IGF1 signalling (IIS) pathways are involved in longevity regulation and are ...
Two insulin receptor mRNA species are expressed in human tissues as a result of alternative splicing...
Impairment of insulin signalling using a combined insulin-fructose treatment protocol over a longer ...
<p>Western blot analysis was used to detect phosphorylated insulin receptor (p-IR) b, total IRb, ins...
<p>(<b>A</b>) The indicated cells were preincubated with the PI-3 kinase inhibitor LY294002 for 1 ho...
<p>A. Relative expression of exogenous <i>Zip7</i> mRNA. B. Relative expression of endogenous and ex...
Mature adipocytes originate from fibroblast-like precursor cells, preadipocytes, which differentiate...
Functional impact of impaired insulin signalling. (A) Human astrocytes treated with ± 1 μM insulin ±...
<p>A: RNA yield (picograms per cell) from the sorted islet cell fractions, expressed as mean + SEM (...
<p>Expression was lower in KO compared to WT astrocytes for all three genes studied. n = 9. ** p<0.0...
<p>Western blot for phosphorylated (P∼p42/44<sup>mapk</sup>) or total (p42/44<sup>mapk</sup>) p42/44...
Reduced IGF1R but not downstream signalling in human astrocytes derived from temporal lobe resection...