Overexpression of <i>TMPRSS2-ERG</i> fusions decreased PSMA expression in LNCaP cells.

<p>A, PSMA expression was detected by real-time PCR in LNCaP cells, normalized to PGD mRNA level. Cells were treated with vehicle DMSO or androgen antagonist flutamide for 2 hours prior to the treatment of the synthetic androgen R1881 for 24 hours. B, TMPRSS2-ERG fusion protein levels were checked by Western blot using anti-V5 antibody. LNCaP cells were transfected with fusion type III, III+72, VI, VI+72, or empty vector for 48 hours. C, Real-time PCR detected PSMA mRNA level in TMPRSS2-ERG fusion-transfected LNCaP cells. Cells were transfected with fusions or empty vector for 48 hours, then treated with or without R1881 for 24 hours. D, ERG expression level in ERG-transfected LNCaP cells by western blot. Cells were transfected with full length ERG for 48 hours. E, PSMA mRNA level in ERG overexpressing LNCaP cell. Cells were treated with or without R1881 for 24 hours post 48 hours of transfection. Experiments were done in triplicate.